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当前位置: 首页 > 产品中心 > Other_reagents > Plantmedia/Rubisco定量试剂盒/30210005-1/1试剂盒
商品详细Plantmedia/Rubisco定量试剂盒/30210005-1/1试剂盒
Plantmedia/Rubisco定量试剂盒/30210005-1/1试剂盒
Plantmedia/Rubisco定量试剂盒/30210005-1/1试剂盒
商品编号: 30210005-1
品牌: plantmedia
市场价: ¥13551.20
美元价: 11518.52
产地: 美国(厂家直采)
公司:
产品分类: 其他试剂
公司分类: Other_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

RubiscoQuantitationKit

ProductInformation

KitContains:

  • RabbitpolyclonalantibodytoRbcL(100µg)
  • Rubisco(RbcL)recombinantproteinstandard(250µl)
  • PEBextractionbuffer(3x2ml)(4Xstock)

Storage(AntibodyandStandard)

  • lyophilized/reconstitutedat-20°C(6months)or-80°C(longtermstorage/years). Pleaseavoidfreezingandthawingofreconstitutedantibodies. Makealiquotsinstead

Storage(Buffer)

  • StableatRTforatleast1month;(shorttermstorage/month)at4°Corlongtermstorage(1year)at20°C.

Background:

  • TherubiscoquantitationkitissuitableforquantitationofRubiscoinplantandalgalsamplesusingquantitativeimmunoblotting. Thekitcontainsanti-RbcLantibody,recombinantRbcLproteinandPEBbufferforquantitativeproteinextraction.

ApplicationInformation

  • Westernblot1:5,000-10,000withstandardECL
  • Expected/apparentMW[kDa]:52.7kDa(ArABIdopsisthaliana),52.5kDa(cyanobacteria),52.3(Chlamydomonasreinhardtii)
  • Confirmedreactivity:Arabidopsisthaliana,Chlamydomonasreinhardtii,Cyanophoraparadoxa,Emilianahuxleyi,Euglenagracilis,Gonyaulaxpolyedra,Heterosigmaakashiwo,Micromonaspusila,Porphyrasp.Spinaciaoleracea,SynechococcusPCC7942,Thalassiosirapseudonana
  • Predictedreactivity:diandmonocots,conifers,mosses,liverworts,welwitschia,greenalgae,redalge,brownalgae,cryptomonad,cyanobacteriaincludingprochlorophytes,gamma-proeobacteria,beta-proteobacteria,alphaproteobacteria
  • Notreactivein:noconfirmedexceptinfrompredictedreactivityknowninthemoment
  • RbcLproteinstandard:at0.15pmoles/µl,amountenoughforgenerationofstandardcurvein85assays(standardcurve:0.0625pmoles,0.125pmoles,0.25pmoles)
  • PEBextractionbuffer:amountenoughtfor48isolationsofplantmaterial(using500µl1xPEBfor100mgfreshweight)or120isolationsofalgalmaterial(using200µl1xPEBforcellamountscorrespondingto4-10ugtotalchlorophyll)

 


  • Storage
    :-20°C
  • BrandName
    :bioPLUS
  • Size
    :1Kit
  • CatalogNo
    :30210005-1
品牌介绍
plant-media是GeneLinx国际公司的一个部门,在1993年于美国成立,其使命是品质的分子工具,生物试剂和分析实验室,教育,研究机构和世界各地的实验室用品。bio-WORLD 专注于生命科学和生物技术产业,Plantmedia是bio-WORLD的重要特色产品系列。bio-WORLD 的总部设在俄亥俄州的都柏林,与位于全球各地的销售办事处。此外,bio-WORLD 提供定制实验室服务的生物技术产业的研发和配方。bio-WORLD是少数可以帮助满足任何组织的多样性供应商的公司。