Plantmedia/Rubisco定量试剂盒/30210005-1/1试剂盒
商品编号:
30210005-1
品牌:
plantmedia
市场价:
¥13551.20
美元价:
11518.52
产品分类:
其他试剂
公司分类:
Other_reagents
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
RubiscoQuantitationKit
ProductInformation
KitContains:
- RabbitpolyclonalantibodytoRbcL(100µg)
- Rubisco(RbcL)recombinantproteinstandard(250µl)
- PEBextractionbuffer(3x2ml)(4Xstock)
Storage(AntibodyandStandard)
- lyophilized/reconstitutedat-20°C(6months)or-80°C(longtermstorage/years). Pleaseavoidfreezingandthawingofreconstitutedantibodies. Makealiquotsinstead
Storage(Buffer)
- StableatRTforatleast1month;(shorttermstorage/month)at4°Corlongtermstorage(1year)at20°C.
Background:
- TherubiscoquantitationkitissuitableforquantitationofRubiscoinplantandalgalsamplesusingquantitativeimmunoblotting. Thekitcontainsanti-RbcLantibody,recombinantRbcLproteinandPEBbufferforquantitativeproteinextraction.
ApplicationInformation
- Westernblot1:5,000-10,000withstandardECL
- Expected/apparentMW[kDa]:52.7kDa(ArABIdopsisthaliana),52.5kDa(cyanobacteria),52.3(Chlamydomonasreinhardtii)
- Confirmedreactivity:Arabidopsisthaliana,Chlamydomonasreinhardtii,Cyanophoraparadoxa,Emilianahuxleyi,Euglenagracilis,Gonyaulaxpolyedra,Heterosigmaakashiwo,Micromonaspusila,Porphyrasp.Spinaciaoleracea,SynechococcusPCC7942,Thalassiosirapseudonana
- Predictedreactivity:diandmonocots,conifers,mosses,liverworts,welwitschia,greenalgae,redalge,brownalgae,cryptomonad,cyanobacteriaincludingprochlorophytes,gamma-proeobacteria,beta-proteobacteria,alphaproteobacteria
- Notreactivein:noconfirmedexceptinfrompredictedreactivityknowninthemoment
- RbcLproteinstandard:at0.15pmoles/µl,amountenoughforgenerationofstandardcurvein85assays(standardcurve:0.0625pmoles,0.125pmoles,0.25pmoles)
- PEBextractionbuffer:amountenoughtfor48isolationsofplantmaterial(using500µl1xPEBfor100mgfreshweight)or120isolationsofalgalmaterial(using200µl1xPEBforcellamountscorrespondingto4-10ugtotalchlorophyll)
- Storage:-20°C
- BrandName:bioPLUS
- Size:1Kit
- CatalogNo:30210005-1
品牌介绍
plant-media是GeneLinx国际公司的一个部门,在1993年于美国成立,其使命是品质的分子工具,生物试剂和分析实验室,教育,研究机构和世界各地的实验室用品。bio-WORLD 专注于生命科学和生物技术产业,Plantmedia是bio-WORLD的重要特色产品系列。bio-WORLD 的总部设在俄亥俄州的都柏林,与位于全球各地的销售办事处。此外,bio-WORLD 提供定制实验室服务的生物技术产业的研发和配方。bio-WORLD是少数可以帮助满足任何组织的多样性供应商的公司。